Dr. Yeoh Kar Kheng

Qualifications: B.Sc. (Hons)(UTM), M.Sc. (UTM), Ph.D. (University of Oxford, England)
Expertise: Organic Synthesis, Small Molecule Inhibitors, Hypoxia Inducible Factor, HIF Prolyl Hydroxylase, Natural Products

(+604)-653 5179
(+604) - 657 4854
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ACADEMIC QUALIFICATIONS
1. DPhil. (University of Oxford, UK)
2. BSc, MSc (Universiti Teknologi Malaysia)

Research Skills

Chemical Organic Synthesis, Mass Spectrometry, Spectrocopy, Chromatography, Extraction of Natural Products, Protein Analysis, Western Blot, Polymerase Chain Reactions.

RESEARCH INTEREST

Chemical Biology (e.g. Hypoxia Inducible Factor (HIF), HIF Proly and Asparaginyl Hydroxylases)
Drug design and synthesis of small-molecule inhibitors.
Natural product chemistry.
Organic Chemistry

RESEARCH TOPICS

Developing of human Hypoxia Inducible Factor (HIF) hydroxylase inhibitors
Developing of Hypoxia Inducible Factor inhibitors
Natural product isolation and purification.

RESEARCH GRANTS

Period	Grant	Amount	Title
1/12/2013-30/11/2016	RUI	RM219,803.00	Synthesis And Evaluation Of Dihydroxybenzoic Acid Analouges As Potential Hypoxia Inducibe Factor (HIF) Prolyl Hydroxylase Inhibitors

THESIS PUBLICATIONS

Yeoh Kar Kheng. “Intermolecular Photoreactions and Selectivity Studies in Confined Space of Y Zeolites”, MSc thesis (2002), University Teknologi Malaysia, Skudai.
Kar Kheng Yeoh. “Studies on the Inhibition of Human Hypoxia Inducible Factor (HIF) Hydroxylases”, DPhil thesis (2012), University of Oxford, United

JOURNAL PUBLICATIONS

2015	S. C. Tan, R. S Gomes, K. K. Yeoh, F. Perbellini, S. Malandraki-Miller, L. Ambrose, L. C. Heather, G. Faggian, C. J Schofield, K. E. Davies, K. Clarke, C. A .Carr, Preconditioning of cardiosphere-derived cells with hypoxia or prolyl-4-hydroxylase inhibitors increases stemness and decreases reliance on oxidative metabolism.Cell transplantation2015 Mar 6. [Epub ahead of print] (ISI, IF:3.13)
2014	R L Chen, O O Ogunshola, K K Yeoh, A. Jani, Mi. Papadakis, S. Nagel, C. J. Schofield, A. M Buchan, HIF Prolyl Hydroxylase Inhibition Prior to Transient Focal Cerebral Ischaemia is Neuroprotective in mice, Journal of neurochemistry, 131 (2), 177-189 (ISI, IF:4.24)
2013	W.S. Aik, M. Demetriades, M. K. K. Hamdan, E. A. L. Bagg, K. K. Yeoh, C. Lejeune, Z. Zhang, M. A. McDonough, C. J. Schofield, Structural Basis for Inhibition of the Fat Mass and Obesity Associated Protein (FTO), Journal of medicinal chemistry, 2013, Article ASAP (ISI, IF:4.80) R Chowdhury, J Ignacio Candela-Lena, M C Chan, D J Greenald, K K Yeoh, Y-M Tian, M A McDonough, A Tumber, N R Rose, A Conejo-Garcia, M Demetriades, S Mathavan,A Kawamura, M K Lee, F v Eeden, et al, Selective Small Molecule Probes for the Hypoxia Inducible Factor (HIF) Prolyl Hydroxylases, ACS Chemical Biology, 2013, 8 (7), pp 1488–1496 (ISI, IF:5.33) R J Hopkinson, A Tumber, C Yapp, R Chowdhury, WS Aik, K H Che, X S Li, J BL Kristensen, O King, M C Chan, K K Yeoh et al, 5-Carboxy-8-hydroxyquinoline is a Broad Spectrum 2-Oxoglutarate Oxygenase Inhibitor which Causes Iron Translocation, Chem. Sci., 2013, 4, 8 (ISI, IF:9.21)
2012	Yeoh, K. K., Chan, M. C., Thalhammer, A., Demetriades, M. Chowdhury, R., Tian, Y.-M., Stolze, I., McNeill, L. A., Lee, M. K., Woon, E. C. Y., Mackeen, M. M., Kawamura, A., Ratcliffe, P. J., MecinoviÄ‡, Dual-action inhibitors of HIF prolyl hydroxylases that induce binding of a second iron ion, Organic & Biomolecular Chemistry, 2012, 11, 732-745 (ISI, IF:3.70) NR Rose, ECY Woon, A. Tumber, LJ Walport, R. Chowdhury, XS Li, ONF King, C Lejeune, SS Ng, T Krojer, MC Chan, AM Rydzik, RJ Hopkinson, KH Che, M. Daniel, KK Yeoh et al, Plant Growth Regulator Daminozide Is a Selective Inhibitor of Human KDM2/7 Histone Demethylases, Journal of Medicinal Chemistry, 2012, 55, 6639-6643( ISI, IF:4.8) Demetriades, M., Leung, I. K., Chowdhury, R., Chan, M. C., McDonough, M. A., Yeoh, K. K., Tian, Y. M., Claridge, T. D., Ratcliffe, P. J., Woon, E. C. Y., Schofield, C. J, Dynamic Combinatorial Chemistry Employing Boronic Acids/Boronate Esters Leads to Potent Oxygenase Inhibitors, Angewandte Chemie International Edition, 2012, 51, 6672-6675 (ISI, IF:13.45) Heather, L. C., Cole, M. A., Tan, J. J., Ambrose, L. J., Pope, S., Abd-Jamil, A. H., Carter, E. E., Dodd, M. S.,Yeoh, K. K., Schofield, C. J., Clarke, K., Metabolic adaptation to chronic hypoxia in cardiac mitochondria, Archive Kreislaufforschung (Basic Res Cardiol), 2012, 107:268, 1-12 (ISI, IF:7.35) Tan, S. C., Carr, C. A., Yeoh, K. K., Schofield, C. J., Davies, K. E., Clarke, K, Identification of valid housekeeping genes for quantitative RT-PCR analysis of cardiosphere-derived cells preconditioned under hypoxia or with prolyl-4-hydroxylase inhibitors, Molecular Biology Reports (Mol Biol Rep), 2012, (ISI, IF:2.93)
2011	S C Tan, K K Yeoh, C A Carr, L C Heather, L Ambrose, J J Tan, C Schofield, K Clarke, Use of prolyl hydroxylase inhibitors to induce HIF-related metabolic changes and increase c-Kit expression in cardiosphere-derived cells, Heart (British Cardiac Society) (Heart), 2011, (ISI, IF:4.22) Chowdhury, R., Yeoh, K. K., Tian, Y-M, Hillringhaus, L., Bagg, E. A., Rose, N. R., Leung, I. K. H., Li, X. S., Woon, E. C. Y., Yang, M., McDonough, M. A., King, O. N., Clifton, I. J., Klose, R. J., C, The Oncometabolite 2-Hydroxyglutarate Inhibits Histone Lysine Demethylases, EMBO reports, 2011, 463-469 (ISI, IF:7.36) Tian, Y-M, Yeoh, K. K., Lee, M. K., Eriksson, T., Kessler, B. M., Kramer, H. B., Edelmann, M. J., Willam, C., Pugh, C. W., Schofield, C. J., and Ratcliffe, P. J., Differential Sensitivity of HIF Hydroxylation Sites to Hypoxia and Hydroxylase Inhibitors, Journal of Biological Chemistry (J Biol Chem), 2011, 286:15, 13041-13051 (ISI, IF:4.77)
2010	Leung, I. K. H., Flashman, E., Yeoh, K. K., and Schofield, C.J., Using NMR Solvent Water Relaxation to Investigate Mettaloenzyme-Ligand Binding Interaction, Journal of Medicinal Chemistry, 2010, 53, 867-875 (ISI, IF:4.80) E. Flashman, S. L. Davies, K. K. Yeoh, C. J. Schofield, Investigating the Dependence of the Hypoxia-Inducible Factor Hydroxylases (Factor Inhibiting HIF and Prolyl Hydroxylase Domain 2) on Ascorbate and Other Reducing Agents, Biochemical Journal (Biochem J), 2010, 427, 135-142 (ISI, IF:4.90)
2009	Stubbs, C., Leonarz, C., Mecinovic, J., Yeoh, K. K., Hindley, N., Lienard, B. M., Sobott, F., Schofield, C. J., and Flashman, E., Application of a proteolysis/mass spectrometry method for investigating the effects of inhibitors on hydroxylase structure, , 2009, 52, ,2799-2805 (ISI, IF:4.80) K. K. Yeoh, T. D. Butters, B. L. Wilkinson, A. J. Fairbanks, Probing Replacement of Pyrophosphate Via Click Chemistry; Synthesis of UDP-Sugar Analogues As Potential Glycosyl Transferase Inhibitors, Carbohydrate research, 2009, 344, 586-591 (ISI, IF:2.03)

Abstract/ Poster Presentation at International Conferences

Yeoh, K. K., Chan, M. C., Thalhammer, A. Demetriades, M. Mecinović, M. Schofield, C. J. “ Dual Action HIF Prolyl Hydroxylase Inhibitors That Induces Binding of Second Iron Ion”. Drug Discovery & Therapy World Congress 2013, June 3rd-6th 2013, Boston, USA.
Yeoh, K. K., Tian, Y-M., Ratcliffe, P. J, Schofield, C. J. “ Differential Sensitivities of HIF Proyl and Asparaginyl Hydroxylation to Chemical Inhibition in Cells”. Drug Discovery & Therapy World Congress 2013, June 3rd-6th 2013, Boston, USA.
Tan, S. C., Carr, C. A. Yeoh, K. K. et al. “Preconditioning Cardiosphere-Derived Cells (CDC) by Hypoxia and Prolyl Hydroxylase Inhibitors (PHI) to Induce HIF-Related Metabolic Changes and C-Kit Expression”. Basic Cardiovascular Sciences 2011 Scientific Sessions, July 18th-21st 2011, New Orleans, LA, USA.
Yeoh, K. K., Tian, Y-M., Schofield, C. J., and Ratcliffe, P. J. “Differential HIF Hydroxylation Sites to Hydroxylase Inhibitors”. Davos Hypoxia Meeting, January 8th-12th 2011, Davos, Switzerland.
Yeoh, K. K., Flashman, E., and Schofield, C. J. “New Methods for the Functional Assignment of 2-OG oxygenases”. Pfizer Poster Competition 2010, Department of Chemistry, University of Oxford.
Leung, I. K. H., Flashman, E., Yeoh, K. K. Schofiled, C. J., and Claridge, T. D. W. “Solvent Relaxation As a Tool to Screen Small Molecule-Metalloprotein Interactions – A Case Study Using Human Proyl Hydroxylase Domain 2”. Small Molecule NMR Conference, September 20th-23rd 2009, Chamonix, France.

HONORS/AWARDS

Year	Award
1999-2002	Dean’s List (Faculty of Sciences, UTM, Skudai)
1999-2002	Kuok Foundation Scholarship ( BSc degree)
2002-2004	National Science Fellowship (MSc degree)
2008-2011	Fellowship of Academic Staff Training Programme (USM) and Government of Malaysia Scholarship (DPhil degree)
2010-2011	Trinity College Graduate Scholarship (Oxford University, UK)